Should We Eat The Meat - 1500 Words

Should we put the Eat in Meat? All over the world, people sit down to a meal that include of steak, pork chops or chicken. It is not too often these days that the consumer considers the life of the animal in which they are eating. It is also not common to even associate the slab of beef on one’s plate with an actual living, breathing, feeling cow. When one hears cow, images of luscious green fields with spotted cows mooing and grazing peacefully fill your mind; and this is how many of our society think cattle are raised for consumption. Instead, cattle are â€Å"raised† in what can be described as holding pens, over crowded stalls with no room to move. Often times, these cows don’t even make it past their first few days of life before being brutally slaughtered. In his article, On Eating Animals, Namit Arora suggests that Americans have something wrong with our way of thinking when it comes to animals. We treat our domesticated pets with love and care as we would a family member; but turn a blind eye to the tortured animals we eat every day. Where do we draw the line on what is okay to do to one living creature and what is considered archaic to do to another? While eating meat is not a crime, or even a bad thing, considering God gave humanity the animals to eat after the floods (Genesis 9, The Bible); is the treatment of said animals something we should consider before we fire up that grill? For many centuries, families raised their own livestock for the primary purpose ofShow MoreRelatedShould Humans Eat Meat? Essay683 Words   |  3 PagesShould humans eat meat Should you, or should you not eat meat. Humans should really eat meat because our body are meant for the meat and it can’t cause cancer and many other health problems. All of the myths on meat are actually people blaming something. Meat are proven to be very nutritious and we are actually evolved to eat meat. It is not just the benefits that people will get from the meat but also get the taste. Thus there is no evidence that suggest a non meat diet will give more healthRead MoreEveryone Shouldnt Be Vegetarian Essay1184 Words   |  5 Pagespounds of chicken and 43.5 pounds of pork per person.(citation?) Only 5% of Americans are vegetarians and with that much meat eaten by Americans, in one year alone why should you to forced to become vegetarian? With meat being the main staple in the Americans diet there is no reason we should take that of the table. That fact alone shows that most Americans’ diets are made up of meat sources. According to the United States Census B ureau (USCB) the U.S. population was 313.8 million; meaning that 16 billionRead MoreShould We Eat Animals? Essay519 Words   |  3 Pages The question if humans should eat meat from animals has been argued over many years. Many people believe that it is wrong and many people believe that it is right. Two out of one hundred Americans are vegetarians (Langley 5). The number is even higher in other places, including India, where thirty-three out of one hundred people are vegetarians (Langley 5). All humans on the Earth should be consuming meat from animals. People who do not eat meat at all can miss out on important nutrientsRead MoreIs It Unethical And Eat Meat?1048 Words   |  5 PagesIs it unethical to eat meat? Essential Writing Skills Is it unethical to eat meat? With many different opinions on the matter, all views need to be considered when discussing and attempting to answer the question. Policies are put in place by institutions (Religious and governmental) that tell us what to eat, what not to eat and how much to eat. All these guidelines raises the question of whose ethics should hold president overall, and what factors do you use to determine this decision. HumankindRead MoreThe Ethics of Eating Meat Essay663 Words   |  3 PagesMeat has been a staple food in the diet of mankind since the early ages of civilization. In the article â€Å"Is Any Meat Good to Eat?† by Sarah Boesveld, she interviews author Jonathan Safran to share his opinion on eating meat and factory farming. He believes that â€Å"...if [people] just ate according to the values they already have, then factory farming would disappear.† Whether or not people realize the sources from which meat in modern day society comes from, they cannot deny the fact that meat is delectableRead MoreEssay on Behind Meat and Meat Factories1404 Words   |  6 Pages Background/History Meat has been in our diet since the start of mankind. We eat meat everyday mindlessly. It is hard to avoid meat since it is everywhere we go. Meat is the majority of today’s food. There are very few vegetarian or vegan options in the food industry. Although, it has been growing more and more popular since it has become a lifestyle. The reason is to be the horrifying truth of today’s meat industry. For those who cannot bear the truth, pick up the vegetarian or vegan lifestyleRead MoreThe Ethics Of Eating Meat Essay1504 Words   |  7 PagesThe Ethics of Eating Meat We are a nation of meat eaters. We are socialized from a young age to consume high levels of animal products. This deeply ingrained meat-eating tradition is a big part of the American standard diet. A visit to the local grocery store shows that there is no shortage of animal products. Isle by isle you see a plethora of meats, neatly packed and ready to be cooked, dairy products neatly shelved, and even candies that contain animal by-products. This is an omnivore’s utopiaRead MoreEssay about Eating Meat1004 Words   |  5 PagesEating Meat Eating meat is part of the daily life of billions of people all over the world. Every day thousands of animals are killed for the production of meat food for people. However, studies have shown that meat is not essential for our existence and gives us nothing more than the other foods on the market. Meat consumption may not harm you or the surrounding environment immediately but there are consequences and sooner or later they will appear. A meatless diet is healthier than a dietRead MoreHow Being Moral Include Animals1261 Words   |  6 Pagesbody, but certain types of food are immensely controversial, such as meat. There are many perspectives for eating food: one states that everyone should be vegan- someone who does not eat any animal products like meat for certain reasons that might be righteous or even health and religious reasons in order for animals, especially cattle, to live life they deserve. Another point of view is that some people believe that eating meat and other animal products do not differ from any other type of food likeRead More Vegetarian Essay example791 Words   |  4 Pagesproblems. Disease. These are all problems caused by being a vegetarian, that is, one who only eats vegetables. There are different degrees of being a vegetarian. To one extreme, is a person who eats nothing associated with animals (no yogurt, ice-cream, or even anything that has come in contact with meat or another animal). The opposite are those who just eat vegan most of the time and will still eat animal bi-products. Then there are others who are in between. Being a vegetarian is not natural

Special Stains Laboratory Report Free Essays

Introduction Staining is a technique that is used to diagnose or study the morphology of abnormal cells such as cancerous cells by highlighting the structural components of a tissue (Bancroft and Gamble, 2008). Staining provides a contrast between different structures in a tissue specimen and allows its examination under a light microscope (Cook, 2006). Haematoxylin and eosin (HE) is a routine stain that is used to microscopically diagnose a vast majority of specimens in which the haematoxylin stains the nuclei, whereas the eosin is used to stain cytoplasm and other extracellular materials (Bancroft and Cook, 1995). We will write a custom essay sample on Special Stains Laboratory Report or any similar topic only for you Order Now According to Slauson and Cooper (2002) special stains are histochemical stains that react with known substances in the tissue. Mohan (2005) explains that special stains are required in various circumstances, where the pathologist needs to demonstrate certain constituent of the cells or the tissue to confirm the diagnosis by etiologic, histogenic and pathogenic components. This technique is called special because they are not a routine stain that is performed on a tissue specimen, instead they are used in addition to HE stained sections (Bancroft and Gamble, 2008). Special stains can identify the presence and abundance of any specific class of molecules in a tissue specimen for example periodic acid-Schiff (PAS) reaction is used to identify carbohydrate substances such as glycogen (Slauson and Cooper, 2002). Other examples include Toluidine blue stain which is used to stain mast cell granules, Perl’s stain demonstrates iron in haemochromatosis, Ziehl-Neelsen stains mycobacteri a and Giemsa staining is used to identify Helicobacter Pylori and Giardia organisms (Slauson and Cooper, 2002 and Bass et al., 2005) Masson’s trichrome (MT) and Congo red are the two main special staining methods used in pathology laboratories. Connective tissues consist of cells such as collagen fibres, elastic fibres, and glycosaminoglycans that are scattered within an extracellular matrix (Starr et al., 2011). These cells are distinguished by using a combination of dyes to stain different structures in various different colours (Starr et al., 2011). Masson’s trichrome is used to express collagen in tissues and involves staining with three different sized dyes to stain three diverse tissue densities (Cook, 2006). MT staining produces three distinct colours as the name suggests; nuclei and other basophilic structures are stained black with iron hematoxylin; collagen is stained green or blue depending on aniline light green or aniline blue; and cytoplasm, muscle, erythrocytes and keratin are stained bright red with Biebrich scarlet stain (Young et al., 2006). Since erythrocytes are the densest as the y are packed with haemoglobin, and less porous tissues they are stained with the smallest dye molecule, the intermediate cytoplasm and muscles cells are stained by the intermediate sized dye and the collagen is stained with the biggest dye (Bancroft and Gamble, 2008). However, it has also been suggested that the acid dye which is the Biebrich Scarlet, first stains the tissue as it binds to its acidophilic elements (Carson, 2001). Subsequently, the tissue is treated with phosphomolybdic/phosphotungstic acids so that the less permeable components retain the red colour, whereas it is diffused out of the collagen fibers causing it to bind with the aniline blue or aniline light green (Bancroft and Gamble, 2008). Young et al., (2006) describes that in addition to the use MT stain in assessing the degree of fibrosis, it is also used to evaluate portal tract structures such as the bile ducts, arteries and veins in inflamed liver According to Romhanyi (1971) (cited in Bely, 2006) Congo red is a special stain that is highly specific and a sensitive method for early diagnosis and recognition of amyloidosis. Cook (2006) states that Congo red is used as the preferred method to identify amyloids in most laboratories on formalin fixed, paraffin embedded tissue of patients with amyloidosis. Kiernan (2007) describes that amyloid is an intercellular material that varies in its composition and is deposited in tissues such as heart, muscle, kidneys, spleen, liver and brain, deposits differ in their composition. Rubin and Strayer (2008) explains that Congo red stain has a linear shaped molecule which helps it to bind to the ? pleated sheet structure of the amyloid through non-polar hydrogen bonds, giving it a red colour. Sen and Basdemir (2003) states that Congo red fluorescence (CRF) is another method that examines the amyloid deposits stained with Congo red under polarized light which shows a red-green birefringence a nd according to Rocken and Eriksson (2009) this is the gold standard for diagnosing amyloid. During this experiment special stain techniques were used to analyse specific tissue elements Aims To identify fibroids in uterine tissue section using Masson’s trichrome stain To identify amyloid in spleen tissue section using Congo red stain To discuss advantages of special stains To use special stains to identify important diagnostic features of the tissue To understand the mechanism used by special stains Method Masson’s Trichrome The formalin fixed and paraffin-embedded uterine tissue section was deparaffinized and rehydrated through 100% alcohol, 95% alcohol, and 70% alcohol. The section was washed in distilled water and then stained in Weigert’s iron hemotoxylin working solution for 10 minutes. It was then rinsed in running warm tap water for 10 minutes and then washed in distilled water. The next step was to stain the uterine tissue section in Biebrich scarlet-acid fuchsin solution for 15 minutes, and then it was washed using distilled water. It was then differentiated in phosphomolybdic – phosphotungstic solution for 15 minutes or until collagen was not red. The tissue section was then transferred directly (without rinsing) to aniline blue solution and stained for 5-10 minutes. Afterwards the tissue section was rinsed briefly in distilled water and differentiated in 1% acetic acid solution for 1 minute. It was then washed in distilled water and dehydrated very quickly through 95% ethyl alco hol, absolute ethyl alcohol (to wipe off Biebrich scarlet-acid fuschin staining) and then cleared in xylene. The section was then mounted with resinous mounting medium. Finally the slide was examined under the light microscope. Congo Red The spleen tissue section was deparaffinized and hydrated to distilled water. The section was then stained in Congo red working solution for 10 minutes and rinsed in distilled water. It was then quickly differentiated (5-10 dips) in alkaline alcohol solution and rinsed in tap water. The section was then counterstained in Gill’s haematoxylin for 10 seconds and rinsed in tap water for 2 minutes. Following that, the section was dipped in ammonia water (made by adding a few drops of ammonium hydroxide to tap water and mixing it well) for 30 seconds or until the sections had turned blue. It was then rinsed in tap water for another 5 minutes and dehydrated through 95% alcohol, and 100% alcohol. The section was cleared in xylene and mounted with mounting medium. The slide was then examined under a light microscope. Fig 1: Normal uterine tissue stained with Masson’s trichrome viewed under 10 x 10 microscopic magnification Fig 2: Fibroid uterine tissue stained with Masson’s trichrome viewed under 10 x 10 microscopic magnification The microscopic slide (Fig. 1) shows a normal uterus tissue that was stained with Masson’s trichrome, which showed the nuclei stained black, smooth muscle stained red and the collagen fibres stained blue. Figure 2 shows a uterus tissue specimen stained with Masson’s trichrome that revealed excessive amount of collagen stained in blue, smooth muscle stained red and nuclei stained black. Fig 3: Spleen tissue stained with Congo red showing amyloid deposits under 10?10 microscopic magnification Fig. 4 Spleen tissue stained with Congo red adapted from Stevens and Lowe, (2000) Discussion Norwitz and Schorge (2006) states that fibroids also referred to as Leiomyomata, are benign tumours of the myometirum of the uterus that is mainly composed of smooth muscle and extracellular fibrous material such as the collagen, Fibroids do not invade surrounding tissues or organs and they can occur in different locations within the uterus (Lark, 1996). The symptoms include heavy periods, frequent urination, constipation, bloating and backache (Tulandi, 2003) Masson’s trichrome was used to stain the uterus tissue (Fig. 1) which showed the collagen stained in blue, smooth muscle and erythrocytes stained red and the nuclei appeared black. MT stain revealed that the normal uterus tissue (Fig. 1) had a small amount of collagen present whereas the other uterus tissue (Fig. 2) showed an elevated amount of collagen stained in blue which suggested fibrosis. MT is an advantageous technique in medicine as it allows comparing the degree of fibrosis before and after the treatment biopsies to show if the treatment has been effective and successful. It is a routine stain for kidney and liver biopsies and this can be used on paraffin fixed sections as well as on frozen sections. Kambic et al., (1986) describes that there are different types of collagen and their organization is better shown using Sirius red with polarized light. Sirius red is a hydrophilic dye, in which type I collagen appears orange or red whereas type III collagen appears green (Kumar, 2005). Congo red was used to stain a spleen tissue section where figure 3 showed amyloid deposits in pink and nuclei in blue. Fig. 4 revealed a high amount of amyloid deposits in pink which suggested amyloidosis that is the disorder caused by abnormal deposition of intracellular or extracellular insoluble amyloid which changes the normal tissue function (Stevens and Lowe, 2000). Special stains is an important tool for pathologists as it allows to the microscopically view and identify cells, tissues and microorganisms, providing an alternative to immunohistochemistry, flow cytometry and various other diagnostic techniques. References Bancroft, J. D. Gamble, M. (2008). ‘Theory and practice of histological techniques’. [Online]. (6th ed). Philadelphia, PA : Churchill Livingstone/Elsevier. Available from: http://books.google.co.uk/books?id=Dhn2KispfdQCprintsec=frontcoverdq=Theory+and+practice+of+histological+techniqueshl=enei=ifGETZKEPcWwhAfSvPW_BAsa=Xoi=book_resultct=resultresnum=1ved=0CC8Q6AEwAA#v=onepageqf=false. [Accessed 7th March 2011]. Bancroft. J. D. Cook, H. C. (1995). ‘Manual of histological techniques and their diagnostic application’. Edinburgh [u.a.] : Churchill Livingstone. Bass, P., Burroughs, S. Way, C. (2005). ‘Systematic pathology : a clinically-orientated core text with self assessment’. Edinburgh : Elsevier Churchill Livingstone. Bely, M. (2006). ‘Histochemical differential diagnosis and polarization optical analysis of amyloid and amyloidosis’ TheScientificWorldJournal. 6, p.154-168. Carson, F. L. (2001). ‘Histotechnology : a self-instructional text’. (2nd ed). Chicago : ASCP Press. Cook, D. J. (2006). ‘Cellular pathology : introduction to techniques and applications’. (2nd ed). Bloxham: Scion Publishing Limited. http://onlinelibrary.wiley.com/doi/10.1046/j.1440-1827.2003.01513.x/abstract Kiernan, J. A. (2007). ‘Histological and histochemical methods’. (4th ed). Cold Spring Harbor (N.Y.) : Cold Spring Harbor Laboratory Press. Mohan, H. (2005). ‘Essential pathology for dental students’. [online]. (3rd ed). New Delhi : Jaypee Brothers. Available from: http://books.google.co.uk/books?id=HmkTtLyxXF8Cprintsec=frontcoverdq=essential+pathology+for+dental+studentshl=enei=xRCCTaiHMs24hAezp6G8BAsa=Xoi=book_resultct=bookthumbnailresnum=1ved=0CDAQ6wEwAA#v=onepageqf=false. [Accessed: 11th March 2011]. Odze, R. D. Goldblum, J. R. (2009). ‘Surgical pathology of the GI tract, liver, biliary tract and pancreas’. [Online]. (2nd ed). Philadelphia, PA : Saunders/Elsevier. Available from: http://books.google.co.uk/books?id=8ITX093f1j0Cpg=PA1143dq=Masson%27s+trichrome+staining+in+liverhl=enei=OB2GTYuVLYuqhAfJ_6m8BAsa=Xoi=book_resultct=book-thumbnailresnum=2ved=0CDgQ6wEwAQ#v=onepageq=Masson%27s%20trichrome%20staining%20in%20liverf=false. [Assessed 16th March 2011] Rocken, C. Eriksson, M. (2009). ‘Amyloid and amyloidoses’ Der Pathologe. 30, (3), p.182-192. Rubin, R. Strayer, D. S. (2008). ‘Rubin’s Pathology : clinicopathologic foundations of medicine’. [Online]. (5th ed). Philadelphia [u.a.] : Lippincott Williams Wilkins. Available from: http://books.google.co.uk/books?id=kD9VZ267wDECpg=PA990dq=mechanism+of+congo+red+stainhl=enei=ADGFTcSQDZGJhQf-n5iuBAsa=Xoi=book_resultct=book-thumbnailresnum=4ved=0CEUQ6wEwAw#v=onepageq=mechanism%20of%20congo%20red%20stainf=false. [Assessed 7th March 2011]. Sen, S. Basdemir, G. (2003). ‘Diagnosis of renal amyloidosis using Congo red fluorescence’ Pathology international. 53, (8), p.534-538. Slauson, D. O. Cooper, B. J. (2002). ‘Mechanisms of disease : a textbook of comparative general pathology’. [Online]. (3rd ed). St. Louis, MO. [u.a.] : Mosby. Available from : http://books.google.co.uk/books?id=vRhtM0UMUh4Cpg=PA5dq=special+stainshl=enei=-vSETaMeqJKEB83RwccEsa=Xoi=book_resultct=book-thumbnailresnum=1ved=0CCoQ6wEwADgK#v=onepageq=special%20stainsf=false. [Accessed 4th March 2011]. Young B., Lowe, J. S, Stevens, A. Heath, J. W. (2006). ‘Wheater’s functional histology : a text and colour atlas’. (5th ed). Edinburgh : Churchill Livingstone Tulandi, T. (2003). ‘Uterine fibroids : embolization and other treatments’. [Online]. Cambridge : Cambridge University Press. Available from: http://books.google.co.uk/books?id=fZ8eha5yIfcCprintsec=frontcoverdq=fibroidshl=enei=oyOGTfnfMJGGhQeY_92_BAsa=Xoi=book_resultct=book-thumbnailresnum=7ved=0CGAQ6wEwBg#v=onepageqf=false. [Assessed 15th March 2011]. Stevens, A. Lowe, J. (2000). ‘Pathology’. (2nd ed). Edinburgh [u. a.] : Mosby Starr, C., Evers, C. A. Starr, L. (2011). ‘Biology : concept and applications’. [Online]. (8th ed). United States : Cengage Learning. Available from: http://books.google.co.uk/books?id=_16xbB2Py_UCpg=PA454dq=connective+tissuehl=enei=zYeITdaEAsywhAf61cm6Dgsa=Xoi=book_resultct=book-thumbnailresnum=10ved=0CFYQ6wEwCTgK#v=onepageq=connective%20tissuef=false. [Assessed 8th March 2011]. Lark, S. M. (1996). ‘Natural treatment of fibroid tumors and endometriosis : effective natural solutions for relieving the heavy bleeding, cramps and infertility that accompany these common female problems’. New Canaan, Conn : Keats Pub. Norwitz, E. R. Schorge, J. O. (2006). ‘Obstetrics and Gynaecology at a glance’. (2nd ed). Malden, Massachusetts : Blackwell. Kumar, R. K. (2005). ‘Morphological methods for assessment of fibrosis’ Methods in Molecular Medicine. 117, p.179-188. Kambic, H. E., Kantrowitz, A. Sung, P. (1986). ‘Vacular graft update : safety and performance, a symposium’. [Online]. Philadelphia, PA : ASTM. Available from: http://books.google.co.uk/books?id=NU0cVwIPk_oCpg=PA162dq=limitations+of+collagen+stainhl=enei=AEKLTcmzMsmYhQe1hJHEDgsa=Xoi=book_resultct=resultresnum=10ved=0CFsQ6AEwCQ#v=onepageqf=false. [Assessed 20th March 2011]. How to cite Special Stains Laboratory Report, Essay examples

Tim Burton Style Analysis free essay sample

For example, one of the beginning scenes in the movie show that the devout woman looks out the window and sees the other woman of the neighborhood flocking together, yet not inviting her to join them. It shows that the woman is an outcast, and is not included in things. Another scene shows Edward’s dejected face and then flashes over to a scene of Kim goofing off with her boyfriend. It lets the viewer understand that Edward is interested romantically in Kim, and thus upset that she is involved with someone else. This is a more subtle technique, and makes the scene more memorable to the viewer because they didn’t come right out and say it. As opposed to telling the audience about something, Burton prefers to let them see for themselves. To show Edwards origins, Burton has Edward looking out the window and has a flashback about how he was created. We will write a custom essay sample on Tim Burton Style Analysis or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Burton also uses setting to a magical extent. Burton is an admirer of over-exaggeration, and this is evident in his distinction of the neighborhood and Edwards’s castle. Whereas the community is bright a cheery, with houses of alternating color and rather small proportions, the castle is dark and opposing. All the houses in the community don’t have more than four feet between them, and even the backyards are visible from the street. Burton also exaggerates the looming castle’s isolation by putting it on a mountain. Burton purposefully designs the community to be the homey, picturesque American dream while Edward’s castle is the definition of gloom and doom. Yet when Peg first comes to the entrance of the castle, she encounters an amazing garden, much more beautiful than anything thing the people in the community could make, showing us that our perceptions aren’t always right. Burton often uses motifs in his movies and Edward Scissorhands is no different. Among many, one of the motifs Burton uses is conformity vs. ndividuality. Burton’s movies all carry common threads, and like all of Burton’s previous main characters, Edward is an outcast. He is a spark of creativity in an otherwise dull society. The citizens of the community choose to conform and the all have flat personalities. One is either a maneater or a motherly type or some other cliche, and that part seems to encompass the rest of their personality. While Edward, who is so different from the way he looks to the way he acts, seems to be the only human character. His personality possesses many layers. The citizens also dress bright and cheery is a retro-style, while Edward with his uncontrollable hair and pale skin looks like a gritty Goth. In conclusion, Burton’s skill shines throughout the entire movie. Using point of view, setting, motifs, and other style techniques, Burton makes Edward Scissorhands a true hit. Tim Burton’s unorthodox style gives new standards to the movie business, and delivers heartwarming stories about misconceptions and finding yourself. With Tim Burton’s masterful skill and playful humor, it becomes obvious why Edward Scissorhands is such a hit. Gigi Orphali 12-8-11 p. 5-6